Submolecular unfolding units of Pseudomonas aeruginosa cytochrome c-551
نویسندگان
چکیده
منابع مشابه
The evolutionary stability of cytochrome c-551 in Pseudomonas aeruginosa and Pseudomonas fluorescens biotype C.
Cytochrome c-551 was prepared from nine different strains of Pseudomonas aeruginosa and six of Pseudomonas fluorescens biotype C, and their amino acid sequences were compared with the sequences previously determined for the cytochromes of type strains of each species. The standard of sequence examination was such that all single amino acid substitutions, delections or insertions ought to have b...
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The homology of Pseudomonas cytochrome c-551 with eukaryotic cytochromes c is examined with a computer-based procedure devised to determine whether similarities exist between these proteins. One method is given by which the more recently evolved cytochromes c might have arisen from the Pseudomonas protein. This procedure involves only common genetic phenomena and accounts for most of the struct...
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The reaction of ascorbate-reduced Pseudomonas cytochrome oxidase with oxygen was studied by using stopped-flow techniques at pH 7.0 and 25 degrees C. The observed time courses were complex, the reaction consisting of three phases. Of these, only the fastest process, with a second-order rate constant of 3.3 X 10(4) M-1.S-1, was dependent on oxygen concentration. The two slower processes were fir...
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The electron-transfer reactions of cellobiose oxidase (CBO) have been investigated by conventional and by rapid-scan stopped-flow spectroscopy at pH 6.0. Analysis of the absorbance/time/wavelength matrix by Singular Value Decomposition (SVD) confirms earlier studies showing that cellobiose rapidly reduces the flavin group (7.7 s-1; cellobiose, 100 microM) which in turn slowly (0.2 s-1) reduces ...
متن کاملCooperative Unfolding Units and Metastable States of Cytochrome c551 from Pseudomonas aeruginosa under Native Conditions
Unfolding pathways of Pseudomonas aeruginosa cytochrome c551 (Pa cyt c) characterized by native state hydrogen-deuterium exchange (NS H/D) methods in conjunction with multi-dimensional NMR techniques have been reported in the literature. Based on the analyses carried out on the H/D exchange data, it has been shown that there are four submolecular unfolding units or three cryptic intermediates (...
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ژورنال
عنوان ژورنال: JBIC Journal of Biological Inorganic Chemistry
سال: 2008
ISSN: 0949-8257,1432-1327
DOI: 10.1007/s00775-008-0370-y